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This further amplifies the release of growth hormone (GH)

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For the detection of lung metastases in mice, specimens were embedded in Tissue-Tek OCT (optimal cutting temperature) compound at 20 C for cryostat sections (6 m), which were mounted on poly-l-lysine-coated coverslips and fixed in acetone for 20 min at 4 C

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The distribution of the GLP-1R is similar to preproglucagon fiber projections with high expression in hypothalamic areas that regulate energy homeostasis, both in rodents and non-human primates (Merchenthaler et al., 1999