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The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)

A specific and diagnostically notable feature of hypothyroidism is myxedema, the accumulation of fatty, mucous deposits, particularly under the eyes, in the hands, and in the feet

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Companies will have to carefully plan elements of trial design, including who to treat, when to treat and end points to use to increase the likelihood of positive findings

10.1016/j.cmet.2021.01.015 15

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