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The treatment was discontinued in 70.4% of patients with cancer diagnosis and in 55% without this disease ( p 12 weeks after recovery), is increasing [402]

Constructs were cloned into a pET21a vector (MilliporeSigma, Burlington, MA) using Gibson Assembly cloning 66 , and plasmids transformed into Rosetta2 cells (MilliporeSigma, Burlington, MA) or BL21 cells (Invitrogen, Carlsbad, CA)

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